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Journal: Cell reports
Article Title: The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells
doi: 10.1016/j.celrep.2025.115401
Figure Lengend Snippet:
Article Snippet: The following primary antibodies were used: mouse anti-β-Actin 1:1000 (Santa Cruz sc-47778), mouse anti-β-tubulin 1:2000 (Sigma-Aldrich T4026), mouse anti-β-dystroglycan 1:500 (Leica Biosystems NCL-β-DG), rabbit anti-Ccm3 1:250 (Proteintech 10294–2-AP), rabbit anti-Cdc42 1:250 (Cell Signaling 2462), rabbit anti-Egr2/Krox20 1:500 (kindly shared by D. Meijer), rabbit anti-GAPDH 1:10,000 (Sigma-Aldrich G9545), rabbit anti-GST tag 1:1000 (Proteintech 80006–1-RR), mouse anti-GST tag 1:1000 (Santa Cruz sc-138), rabbit anti-His tag 1:1000 (Cell Signaling 2365S), rabbit anti-His tag 1:50,000 (Cell Signaling 2366T), goat anti-integrin α6 1:250 (Santa Cruz sc-6597), rabbit anti-integrin b1 1:250 (Cell Signaling 4706), rat anti-integrin b4 1:250 (Abcam ab25254), mouse anti-Mob4/Phocein 1:250 (Santa Cruz sc-137229), rabbit anti-Mst1 1:500 (Cell Signaling 3682), rabbit anti-Mst2 1:500 (Abcam ab52641), rabbit anti-p-Mst1/2 1:250 (Proteintech 28953–1-AP), rabbit anti-NF2 1:500 (Cell Signaling 6995), rabbit anti-p-NF2 1:500 (Cell Signaling 1328), rabbit anti-Oct6/Pou3f1 1:500 (kindly shared by D. Meijer), rabbit anti-Pak1 1:500 (Cell Signaling 2602), rabbit anti-p-Pak1 1:500 (Cell Signaling 2601), mouse anti-Rac1 1:250 (EMD Millipore 05–389), mouse anti-Rac1 1:250 (Cytoskeleton, Inc. ARC03), rabbit anti-Rac1 1:500 (Thermo Fisher Scientific PA1–091), rabbit anti-Rac1 1:500 (Proteintech 24072–1-AP), rabbit anti-Sox10 1:250 (Cell Signaling 89356), mouse anti-StrepII tag 1:250 (Thermo Fisher Scientific MA5–37747), mouse anti-STRIP1 1:250 (Origene TA502314), mouse anti-striatin-1 (Santa Cruz sc-136084), rabbit anti-striatin-3 1:100 (Atlas Antibodies HPA004636),
Techniques: Generated, Virus, Recombinant, SYBR Green Assay, Protease Inhibitor, Plasmid Preparation, In Situ, BIA-KA, Mutagenesis, Software